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Genome Database of the Endemic Fungal Pathogen Pythium Insidiosum for Exploration of Microbial Biodiversity and Hypothetical Proteins that Lead to the Discovery of Novel Cell Process, Pathogenicity and Diagnostic and Therapeutic Markers

ธีรพงษ์ กระแจะจันทร์; Theerapong Krajaejun; วีรยุทธ กิตติโชติรัตน์; Weerayuth Kittichotirat; ปรีชา ปทุมเจริญผล; Preecha Patumcharoenpol; สิทธิโชค ตั้งภัสสรเรือง; Sithichoke Tangphatsornruang; ธิดารัตน์ รุจิรวรรธน์; Thidarat Rujirawat; ภัทรนา แซ่จิว; Pattarana Sae-Chew; ดวงดาว วิชาดากุล; Duangdao Wichadakul; ชมพูเนกข์ ยุรญาติ; Chompoonek Yurayart;
Date: 2567-09
Abstract
Pythium insidiosum is a pathogenic oomycete that infects humans and animals, causing a fatal infectious disease, pythiosis. The number of reported pythiosis cases is increasing worldwide, particularly in Thailand, where it is endemic. However, diagnosing and treating pythiosis is challenging due to the lack of effective antifungal drugs or vaccines. Surgery to remove the infected organ is often necessary, but many patients do not survive the infection. To gain a deeper understanding of P. insidiosum and potentially discover new ways to detect and control the pathogen, it is crucial to study its basic biology, diversity, evolution, and pathogenesis. The genome of an organism contains vital information that distinguishes it from others. Thus, a logical approach is to sequence and compare the genomes of P. insidiosum and related species to identify genetic differences that contribute to their phenotypic diversity, including traits like virulence and drug resistance. In this study, we used the MGIseq2000 next-generation sequencing platform to sequence the genomes of 27 P. insidiosum strains isolated from humans, animals, and the environment. Additionally, we included the existing sequenced genomes to provide the most comprehensive dataset of 75 genomes of this pathogen. We developed our in-house bioinformatic software, "Gene Table," to facilitate comparative genomic analyses. This allowed us to identify the core and species-specific genes of P. insidiosum. By conducting a phylogenomic analysis of the core genes, we gained insights into its evolution. We also identified hypothetical protein-encoding genes that could be potential targets for future biology studies and clinical applications. In summary, this project sequenced 27 P. insidiosum genomes and combined them with available genome data, resulting in the most comprehensive genome database of this pathogen. We developed an online comparative genomic analysis tool called "Pythium Gene Table" to facilitate genomic comparison of these 101 oomycetes. Analyzing the genome contents of various P. insidiosum strains and related species enhanced our understanding of their biology, pathogenicity, and evolution. Many hypothetical protein-encoding genes have been identified, some of which could serve as new diagnostic and therapeutic markers. Additionally, this research project has fostered a research network among scientists from various Thai institutions and is expected to lead to at least one international publication in the near future.
Copyright ผลงานวิชาการเหล่านี้เป็นลิขสิทธิ์ของสถาบันวิจัยระบบสาธารณสุข หากมีการนำไปใช้อ้างอิง โปรดอ้างถึงสถาบันวิจัยระบบสาธารณสุข ในฐานะเจ้าของลิขสิทธิ์ตามพระราชบัญญัติสงวนลิขสิทธิ์สำหรับการนำงานวิจัยไปใช้ประโยชน์ในเชิงพาณิชย์
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