Abstract
Peripheral arterial disease (PAD) is commonly caused by atherosclerosis. PAD is one of the major health problems in the modern society. Most patients who suffer from diabetes mellitus type II tend to suffer also from a severe PAD complication, i.e., critical limb ischemia (CLI). The main therapeutic approach for CLI is either surgical debridement or endovascular revascularization in order to improve blood flow to the affected body area, like extremity. However, approximately 20-30% of patients with CLI are not able to receive vascular or endovascular procedures and amputation appears to be the only option. This is to say that CLI patients have a relatively high tendency to have their afflicted body parts removed and the mortality rate of this group of patients is also high. With severe obstruction of blood flow, type II diabetic patients often have their limb loss from debridement. To search for the new therapeutic option of PAD/CLI, most of the researchers are focusing on progenitor cells, which were identified as a potential new therapeutic option to induce therapeutic angiogenesis. Recently, cell-based therapy for treating CLI has been introduced. Several studies show that the cell-based therapy for vascular regenerations is safe and effective. Especially, the new culture medium called culture medium which able to enhance quality and quantity mononuclear cell. The recent study reveals the efficacy of QQMNC in angiogenesis and vasculogenesis function already. This study was conducted to further investigate the efficacy and safety in animal model. The QQMNCs were injected to the critical ischemic limb mice model at the site of occlusion intramuscularly, then, the blood perfusion was assessed by using Laser doppler image scan. In this study, the group of nude mice which was injected with QQMNC show a promising angiogenesis and vaslucogenesis significantly while the group of PBMNC injection was not. The PBMNC injection group show gangrene and finally lost their limb. This study show the efficacy and safety in animal model which will pave the way to the next phase of the study about applying this method of QQMNC for treating in critical limb ischemic patient.
